Investigations funded by the Longer Life Foundation have resulted to date in 167 articles in peer-reviewed medical journals. Find them here.
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2022 Publications

  • Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism

    Zhang et al., 2022, Cell Reports Medicin3, 100498

  • Insulin, Nobel laureates and The
    Journal of Physiology

    Bettina Mittendorfer, Center for Human Nutrition, Washington
    University School of Medicine, St Louis,
    MO, USA
    , J Physiol 0.0 (2022) pp 1–2, 2022

  • Txnip Enhances Fitness of Dnmt3a-Mutant
    Hematopoietic Stem Cells via p21

    Christine R. Zhang, Grant A. Challen, et al; Blood Cancer Discovery,, Published online May, 2022.                                                                                                                                                                                                                       

  • Persisting uropathogenic Escherichia coli lineages
    show signatures of niche-specific within-host
    adaptation mediated by mobile genetic elements

    Thanert et al., 2022, Cell Host & Microbe 30, 1–14
    July 13, 2022 ª 2022 Elsevier Inc.

2021 Publications

  • Evaluating the therapeutic potential of ADAR1 inhibition for triple-negative breast cancer

    Che-Pei Kung 1, Kyle A. Cottrell1, Sua Ryu1, Emily R. Bramel1, Raleigh D. Kladney1, Emily A. Bao1, Eric C. Freeman1, Thwisha Sabloak1,  Leonard Maggi Jr.1,  Jason D. Weber 1,2: Oncogene

  • PARROT: a flexible recurrent neural network framework for analysis of large protein datasets

    Daniel Griffith1,2, Alex S. Holehouse1,2* 1.
    bioRxiv, Cold Spring Harbor Laboratory.                                                                                                                                                                                                                                                                       


  • Txnip Enhances Fitness of Dnmt3a-Mutant Hematopoietic Stem Cells via p21

    Christine R. Zhang1, Elizabeth L. Ostrander1, Ostap Kukhar1, Cates Mallaney1, Jiameng Sun1,
    Emily Haussler1, Hamza Celik1, Won Kyun Koh1, and Grant A. Challen1; et al
    American Assoc. for Cancer Research. Published online May 2022. Blood Cancer Discovery.                                                                                                          

  • Associations Among Adipose Tissue Immunology, Inflammation, Exosomes and Insulin Sensitivity in People With Obesity and Nonalcoholic Fatty Liver Disease

    Anja Fuchs,1 Dmitri Samovski,2 Gordon I. Smith,2 Vincenza Cifarelli,2 Sarah S. Farabi,2 Jun Yoshino,2 Terri Pietka,2 Shin-Wen Chang,1 Sarbani Ghosh,1 Terence M. Myckatyn,1 and Samuel Klein2
    Gastroenterology 2021;161:968–981

  • The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA

    Cubuk J, Alston JJ, Incicco JJ, Singh S, Stuchell-Brereton MD, Ward MD, Zimmerman MI, Vithani N, Griffith D, Wagoner JA, Bowman GR, Hall KB, Soranno A, Holehouse AS., Nat Comun, 2021.                                                                                                                                                                                                                                                                       



  • Preeclampsia Predicts Risk of Hospitalization for Heart Failure With Preserved Ejection Fraction

    Dominique Williams, MD,a Molly J. Stout, MD, MSCI,b Joshua I. Rosenbloom, MD, MPH,c Margaret A. Olsen, PHD, MPH,d Karen E. Joynt Maddox, MD, MPH,a Elena Deych, MS,a Victor G. Davila-Roman, MD,Kathryn J. Lindley, MDa,c
    Journal of the American College of Cardiology. Published online 2021. Vol. 78, No. 23.                                                                                                                                                                                                                                                                         

  • Beta-cell function and plasma insulin clearance in people with
    obesity and different glycemic status

    Bettina Mittendorfer, … , Mihoko Yoshino, Samuel Klein: J Clin Invest. 2021.

2020 Publications

  • Microbiome Restoration by RBX2660 Does Not Preclude Recurrence of Multidrug-Resistant Urinary Tract Infection Following Subsequent Antibiotic Exposure: A Case Report

    Eric C. Keen, Preston Tasoff, Tiffany Hink, Kimberly A. Reske, Carey-Ann D. Burnham, Gautam Dantas, Jennie H. Kwon, and Erik R. Dubberke; CDC Prevention Epicenters Program, Open Forum Infectious Diseases

  • Amended: High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy

    Xiangyu Zhang, Ismail Sergin, Trent D. Evans, Se-Jin Jeong, Astrid Rodriguez-Velez, Divya Kapoor, Sunny Chen, Eric Song, Karyn B. Holloway, Jan R. Crowley, Slava Epelman, Conrad C. Weihl, Abhinav Diwan, Daping Fan, Bettina Mittendorfer, Nathan O. Stitziel, Joel D. Schilling, Irfan J. Lodhi, Babak Razani; Nat Metab. 2020 January ; 2(1): 110–125. doi:10.1038/s42255-019-0162-4.

  • Divergent Effects of Dnmt3a and Tet2 Mutations on Hematopoietic Progenitor Cell Fitness

    Elizabeth L. Ostrander, Ashley C. Kramer, Cates Mallaney, Hamza Celik, Won Kyun Koh, Jake Fairchild, Emily Haussler, Christine R.C. Zhang, and Grant A. Challen;
    Stem Cell Reports. April 14, 2020; Vol. 14: 551–560.                                                                                                                                                                                                                                                                                                                                                                

  • Lactotrehalose, an Analog of Trehalose, Increases Energy Metabolism Without Promoting Clostridioides difficile Infection in Mice

    Yiming Zhang, Nurmohammad Shaikh, Jeremie L. Ferey, Umesh D. Wankhade, Sree V. Chintapalli, Cassandra B. Higgins, Jan R. Crowley, Monique R. Heitmeier, Alicyn I. Stothard, Belgacem Mihi, Misty Good, Takanobu Higashiyama, Benjamin M. Swarts, Paul W. Hruz, Kartik Shankar, Phillip I. Tarr, and Brian J. DeBosch; Gastroenterology 2020, Vol. 158, No. 5;158:1402–1416

  • What are protective antibody responses to pandemic SARS-CoV-2?

    Jeffrey P. HendersonJ Clin Invest. 2020;130(12):6232–6234. Published in Volume 130, Issue 12, Dec. 1, 2020.

  • Association of Convalescent Plasma Therapy With Survival in Patients With Hematologic Cancers and COVID-19

    Michael A. Thompson, MD, PhD1; Jeffrey P. Henderson, MD, PhD2; Pankil K. Shah, MD, MSPH3; et al
    JAMA Oncol. Published online June 17, 2021. doi:10.1001/jamaoncol.2021.1799.                                                                                                                                                                                                                                                                             

  • Dietary protein intake and obesity-associated cardiometabolic function

    Alan Fappi 1, Bettina Mittendorfer; Curr Opin Clin Nutr Metab Care . 2020 Nov;23(6):380-386. doi: 10.1097/MCO.0000000000000689.

  • High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy

    Xiangyu Zhang, Ismail Sergin, Trent D. Evans, Se-Jin Jeong, Astrid Rodriguez-Velez, Divya Kapoor, Sunny Chen, Eric Song, Karyn B. Holloway, Jan R. Crowley, Slava Epelman, Conrad C. Weihl, Abhinav Diwan, Daping Fan, Bettina Mittendorfer, Nathan O. Stitziel, Joel D. Schilling, Irfan J. Lodhi & Babak Razani Nature Metabolism . 23 January 2020. Volume 2, pages 110–125 (2020)

2019 Publications

  • Young versus old age at diagnosis confers distinct genomic profiles
    in patients with polycythemia vera

    Fowles JS,1 How J,1 Allen MJ,1 Oh ST.1
    Leukemia (2019) 33:1522–1526

  • Comparative Genomics of Antibiotic-Resistant Uropathogens Implicates Three Routes for Recurrence of Urinary Tract Infections

    Thänert R, Reske KA, Hink T, Wallace TA, Wang B, Schwartz DJ, Seiler S, Cass C, Burnham CD, Dubberke ER, Kwon JH, Dantas G, for the CDC Prevention Epicenter Program Clinical Science and Epidemiology (2019) e01977-19

  • Intermittent Fasting Confers Protection in CNS Autoimmunity by Altering the Gut Microbiota 

    Cignarella F, Cantoni C, Ghezzi L, Salter A, Dorsett Y, Chen L, Phillips D, Weinstock GM, Fontana L, Cross AH, Zhou Y, Piccio L. 
    Cell Metabolism. 2018 Jun 5; 27(6): 1222-35. 

  • Higher Body Mass Index Is Associated with Lower Cortical Amyloid-β Burden in Cognitively Normal Individuals in Late-Life

    Vineeth Thirunavu, Austin McCullough, Yi Suc, Shaney Flores, Aylin Dincer, John C. Morris, Carlos Cruchaga, Tammie L.S. Benzinger, Brian A. Gordon; 
    J Alzheimers Dis. 2019 ; 69(3): 817–827. doi:10.3233/JAD-190154.

  • Maximal dose-response of vitamin-K2 (menaquinone-4) on undercarboxylated osteocalcin in women with osteoporosis

    Tusar K Giri1, David Newton, Opal Chaudhary, Elena Deych, Nicola Napoli, Reina Armamento-Villareal1, Kathy Diemer, Paul E Milligan, and Brian F Gage; Int J Vitam Nutr Res (2019), 1–7

  • Posttranscriptional modulation of TERC by PAPD5 inhibition rescues hematopoietic development in dyskeratosis congenita

    Wilson Chun Fok, Siddharth Shukla, Alexandre Teixeira Vessoni, Kirsten Ann Brenner, Roy Parker, Christopher M. Sturgeon, and Luis Francisco Zirnberger Batista; Blood Journal: The American Society of Hematology. 21 March 2019, Vol. 133, No. 12  

2018 Publications

  • Health Benefits of the Mediterranean Diet: Metabolic and Molecular Mechanisms

    Tosti V, Bertozzi B, Fontana L. 
    Journal of Gerontology: Biological Sciences. 2018 Mar 2; 73(3):318-26. 

  • The effects of graded caloric restriction: XII. Comparison of mouse to human impact on cellular senescence in the colon  

    Fontana L, Mitchell SE, Wang B, Tosti V, van Vliet T, Veronese N, Bertozzi B, Early DS, Maissan P, Speakman JR, Demaria M. 
    Aging Cell 2018 Jun; 17(3): e12746. 

  • Intermittent Fasting Confers Protection in CNS Autoimmunity by Altering the Gut Microbiota 

    Cignarella F, Cantoni C, Ghezzi L, Salter A, Dorsett Y, Chen L, Phillips D, Weinstock GM, Fontana L, Cross AH, Zhou Y, Piccio L. 
    Cell Metabolism. 2018 Jun 5; 27(6): 1222-35. 

  • Interventions to promote cardiometabolic health and slow cardiovascular ageing 

    Fontana L. Nature Reviews Cardiology. 2018 Sep; 15(9): 566-77. 

  • Calorie restriction induces reversible lymphopenia and lymphoid organ atrophy due to cell redistribution  

    Contreras NA, Fontana L, Tosti V, Nikolich-┼Żugich J. 
    Geroscience. 2018 Jun; 40(3): 279-91. 

  • Low-protein diet in cancer: ready for prime time? 

    Pili R, Fontana L. Nature Reviews Endocrinology. 2018 May 15; 14(7): 384-6.

  • Caloric Restriction and Cellular Senescence: Mechanisms of Ageing and Development

    Fontana L, Nehme J, Demaria M. 
    Mechanisms of Ageing and Development. 2018 Dec; 176: 19-23. 

  • Sarcosine is Uniquely Modulated by Aging and Dietary Restriction in Rodents and Humans

    Walters RO, Arias E, Diaz A, Burgos ES, Guan F, Tiano S, Mao K, Green CL, Qiu Y, Shah H, Wang D, Hudgins AD, Tabrizian T, Tosti V, Shechter D, Fontana L, Kurland IJ, Barzilai N, Cuervo AM, Promislow DEL, Huffman DM. 
    Cell Reports. 2018 Oct 16; 25(3): 663-76. 

  • Dietary protein restriction reduces circulating VLDL triglyceride levels via CREBH-APOA5-dependent and -independent mechanisms

    Treviño-Villarreal JH, Reynolds JS, Bartelt A, Langston PK, MacArthur MR, Arduini A, Tosti V, Veronese N, Bertozzi B, Brace LE, Mejia P, Trocha K, Kajitani GS, Longchamp A, Harputlugil E, Gathungu R, Bird SS, Bullock AD, Figenshau RS, Andriole GL, Thompson A, Heeren J, Ozaki CK, Kristal BS, Fontana L, Mitchell JR. 
    JCI Insight. 2018 Nov 2; 3(21). 

  • Prophylactic probiotics for cancer therapy-induced diarrhoea: a meta-analysis

    Wardill HR, Van Sebille YZA, Ciorba MA, Bowen JM.
    Curr Opin Support Palliat Care. 2018 Mar 14. 

  • Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling

    Dmitri Samovski, Pallavi Dhule, Terri Pietka, Miriam Jacome-Sosa, Eric Penrose, Ni-Huiping Son, Robert C. Flynn, Kooresh I. Shoghi, Krzysztof L. Hyrc, Ira J. Goldberg, Eric R. Gamazon and Nada A. Abumrad
    Diabetes 2018 May; db171226.

  • Dietary Protein Restriction Reprograms Tumor-Associated Macrophages and Enhances Immunotherapy

    Orillion A, Damayanti NP, Shen L, Adelaiye-Ogala R, Affronti H, Elbanna M, Chintala S, Ciesielski M, Fontana L, Kao C, Elzey BD, Ratliff TL, Nelson DE, Smiraglia D, Abrams SI, Pili R. 
    Clinical Cancer Research. 2018 Dec 15; 24(24): 6383-95. 

  • Trends in Age-Related Disease Burden and Healthcare Utilization 

    Atella V, Piano-Mortari A, Kopinska J, Belotti F, Lapi F, Cricelli C, Fontana L.
    Aging Cell 2018; 18(1): e12861. 

  • Acute graft-versus-host disease following lung transplantation in a patient with a novel TERT mutation

    Jonathan R Brestoff, Alexandre T Vessoni, Kirsten A Brenner,
    Geoffrey L Uy, John F DiPersio, Morey Blinder, Chad A Witt, Derek E Byers, Ramsey R Hachem, Elbert P Truclock, Dayna S Early, Milan J Anadkat, Amy Musiek, Cylen Javidan-Nejad, Dennis M Balfe, Ilana S Rosman, Chang Liu, Lingxin Zhang, George J Despotis, Marianna B Ruzinova, Jennifer K Sehn,Ina Amarillo, Jonathan W Heusel, Wojcieh Swat, Brian S Kim, Lukas D Wartman, Roger D Yusen, Luis F Z Batista;

    Thorax 2018;73:489–492. doi:10.1136/thoraxjnl-2017-211121. 

2017 Publications

  • The science of nutritional modulation of aging

    Fontana L.
    Ageing Research Reviews. 2017 Oct; 39: 1-2.

  • Perspective: Improving Nutritional Guidelines for Sustainable Health Policies: Current Status and Perspectives

    Magni P, Bier DM, Pecorelli S, Agostoni C, Astrup A, Brighenti F, Cook R, Folco E, Fontana L, Gibson RA, Guerra R, Guyatt GH, Ioannidis JP, Jackson AS, Klurfeld DM, Makrides M, Mathioudakis B, Monaco A, Patel CJ, Racagni G, Schünemann HJ, Shamir R, Zmora N, Peracino A. 
    Advances in Nutrition. 2017 Jul 14; 8(4): 532-45. 

  • In a Randomized Trial in Prostate Cancer Patients, Dietary Protein Restriction Modifies Markers of Leptin and Insulin Signaling in Plasma Extracellular Vesicles

    Eitan E, Tosti V, Suire CN, Cava E, Berkowitz S, Bertozzi B, Raefsky SM, Veronese N, Spangler R, Spelta F, Mustapic M, Kapogiannis D, Mattson MP, Fontana L. 
    Aging Cell. 2017 Dec; 16(6): 1430-3. 

  • Beyond Calories: An Integrated Approach to Promote Health, Longevity, and Well-Being. Gerontology

    Bertozzi B, Tosti V, Fontana L.
    Gerontology. 2017; 63(1): 13-9. (Epub 2016 May 14.)

  • Alterations in 3-Hydroxyisobutyrate and FGF21 Metabolism are Associated with Protein Ingestion-Induced Insulin Resistance 

    Harris LLS, Smith GI, Patterson BW, Ramaswamy RS, Okunade A, Kelly SC, Porter LC, Klein S, Yoshino J, Mittendorfer B:
    Diabetes 2017 (PMID: 28473464) 

  • Dietary protein, aging and nutritional geometry

    Simpson SJ, Le Couteur DG, Raubenheimer D, Solon Biet SM, Cooney GJ, Cogger VC, Fontana L.
    Aging Research Reviews. 2017 Oct; 39:78-86. 

  • Calorie Restriction in Humans: An Update

    Most J, Tosti V, Redman LM, Fonana L.
    Aging Research Reviews 2017 Oct; 39: 36-45. (Epub 2016 Aug 17.)

  • Renal and Systemic Effects of Calorie Restriction in Type 2 Diabetes With Abdominal Obesity: A Randomized Controlled Trial

    Ruggenenti P, Abbate M, Ruggiero B, Rota S, Trillini M, Aparicio C, Parvanova A, Iliev IP, Pisanu G, Perna A, Russo A, Diadei O, Martinetti D, Cannata A, Carrara F, Ferrari S, Stucchi N, Remuzzi G, Fontana L.  
    Diabetes. 2017 Jan; 66(1): 75-86. (Epub 2016 Sep 15.)

  • Gnotobiotic Mouse Models for Identifying Consistent Effects of Different Nutritional Lifestyles on the Gut Microbiota of Multiple Unrelated Humans

    Griffin NW, Ahern PP, Cheng J, Heath AC, Fontana L, Gordon JI.
    Cell Host and Microbe. 2017: 21(1): 84-96.

  • A Simple Culture System for Long-Term Imaging of Individual C. Elegans

    Pittman WE, Sinha DB, Zhang WB, Kinser HE & Pincus Z.
    Lab Chip. 2017; 77, 71 (2017)(open access available 2018-11-07.)

  • Short-term consumption of a plant protein diet does not improve glucose homeostasis of young C57BL/6J mice

    Lamming DW, Baar EL, Arriola Apelo SI, Tosti V, Fontana L. 
    Nutrition and Healthy Aging. 2017 Dec 7; 4(3):239-45.

2016 Publications

  • Successful Completion of the Pilot Phase of a Randomized Controlled Trial Comparing Sentinel Lymph Node Biopsy to No Further Axillary Staging in Patients with Clinical T1-T2 N0 Breast Cancer and Normal Axillary Ultrasound

    Cyr AE, Tucker N, Ademuyiwa F et al. J Am Coll Surg.2016 Aug;223(2):399-407.

  • Higher chylomicron remnants and LDL particle numbers associate with CD36 SNPs and DNA methylation sites that reduce CD36

    Love-Gregory L, Kraia AT, Allum F et al. J Lipid Res. 2016 Dec57(12):2176-84.

  • Extended Twilight among Isogenic C. elegans Causes a Disproportionate Scaling between Lifespan and Health

    Zhang WB, Sinha DB, Pittman WE, Hvatum E, Stroustrup N, Pincus Z.
    Cell Systems, 2016 Oct 26;3(4):333-45.

  • NAMPT-Mediated NAD(+) Biosynthesis in Adipocytes Regulates Adipose Tissue Function and Multi-organ Insulin Sensitivity in Mice

    Stromsdorfer KL, Yamaguchi S, Yoon MJ, Moseley AC, Franczyk MP, Kelly SC, Qi N, Imai S, Yoshino J. Cell Reports. 2016 Aug 16;16(7):1851-60.


  • Cerebrospinal Fluid Biomarkers and Reserve Variables as Predictors of Future "Non-Cognitive" Outcomes of Alzheimer's Disease

    Ingber AP, Hassenstab J, Fagan AM, Benzinger TL, Grant EA, Holtzman DM, Morris JC, Roe CM. J Alzheimers Dis. 2016;52(3):1055-64.

  • Human Metabolome-Derived Cofactors are Required for the Antibacterial Activity of Siderocalin in Urine

    Shields-Cutler RR, Crowley JR, Miller CD, Stapleton AE, Cui W, Henderson JP.
    The Journal of Biological Chemistry. 2016 Oct. 25.

  • Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle

    Yang L, Licastro D, Cava E, Veronese N, Spelta F, Rizza W, Bertozzi B, Villareal DT, Hotamisligil GS, Holloszy JO, Fontana L. Cell Reports. 2016 Jan 26;14(3):422-8.


  • Decreased Consumption of Branched-Chain Amino Acids Improves Metabolic Health

    Fontana L, Cummings NE, Arriola Apelo SI et al.  Cell Reports, 2016 Jul 12;16(2):520-30.


  • Calorie restriction in humans: An update

    Most J, Tosti V, Redman LM, Fontana L.
    Ageing Res Rev., 2016 Aug 17.


  • High-protein intake during weight-loss therapy eliminates the weight loss-induced improvement in insulin action in obese postmenopausal women

    Smith GI, Yoshino J, Kelly SC, Reeds DN, Okunade A, Patterson BW, Klein S, Mittendorfer B. Cell Reports. Oct. 11, 2016. 

  • Predicting all-cause mortality from basic physiology in the Framingham Heart Study

    Zhang WB, Pincus Z. Aging Cell, 2016 Feb 15. (1):39-48.

  • Effects of moderate and subsequent progressive weight loss on metabolic function and adipose tissue biology in humans with obesity

    Magkos F, Fraterrigo G, Yoshino J, Luecking C, Kirbach K, Kelly SC, de Las Fuentes L, He S, Okunade AL, Patterson BW, Klein S.
    Cell Metabolism, 2016 Feb 22. pii: S1550-4131(16)30053-5. doi: 10.1016/j.cmet.2016.02.005. [Epub ahead of print]

  • Probiotic Gut Bacteria Enhance Cancer Immunotherapy in a Mouse Model of Melanoma

    Dey N, Ciorba MA.
    Gastroenterology. 2016 May 26. pii: S0016-5085(16)34458-4. doi: 10.1053/j.gastro.2016.05.015. [Epub ahead of print] No abstract available.

  • Long-Term Administration of Nicotinamide Mononucleotide Mitigates 
    Age-Associated Physiological Decline in Mice

    Kathryn F. Mills, Shohei Yoshida, Liana R. Stein, ..., Koji Uchida,
    Jun Yoshino, Shin-ichiro Imai
    Cell Metabolism, December 13, 2016 , 24, 1–12 [Epub ahead of print]

  • Combined associations of body weight and lifestyle factors with all-cause and cause-sepcific mortality in men and women: prospective cohort study

    Veronese N, Li Y, Manson JE, Willett WC, Fonana L, Hu FB.
    BMJ 2016 Nov. 24; 355.

2015 Publications

  • Adipose tissue monomethyl branched-chain fatty acids and insulin sensitivity: Effects of obesity and weight loss

    Su X, Magkos F, Zhou D, Eagon JC, Fabbrini E, Okunade AL, Klein S.
    Obesity, 2015 Feb; 23(2):329-34


  • Urine aquaporin 1 and perilipin 2 differentiate renal carcinomas from other imaged renal masses and bladder and prostate cancer

    Morrissey JJ, Mobley J, Figenshau RS, Vetter J, Bhayani S, Kharasch ED
    Mayo Clinic Proceedings, 2015 Jan; 90(1):35-42


  • Human urinary composition controls antibacterial activity of siderocalin

    Shields-Cutler RR, Crowley JR, Hung CS, Stapleton AE, Aldrich CC, Marschall J, Henderson JP
    The Journal of Biological Chemistry, 2015 April 10, 

  • Promoting health and longevity through diet: from model organisms to humans

    Fontana L, Partridge L
    Cell, 2015 March 25; 161(1); 106-18

  • Calorie restriction, endothelial function and b¬lood pressure homeostasis

    Spelta F, Bertozzi B, Cominacini L, Fontana L
    Vascul Pharmacol, 2015 Feb-Mar, 65-6:1-2.

  • Low protein intake is associated with a major reduction in IGF-1, cancer, and overall mortality in the 65 and younger but not older population

    Levine M, Suarez J, Brandhorst S, Balasubramanian, Cheng CW, Madia F, Fontana L, Mirisola MP, Wan J, Passarino G, Kennedy BK, Wei M, Cohen P, Crimmins E, Longo VD
    Cell Metabolism 2014 Mar 4;19(3):407–17

  • Probiotics to prevent gastrointestinal toxicity from cancer therapy: an interpretive review and call to action

    Ciorba MA, Hallemeier CL, Stenson WF, Parikh PJ.
    Curr Opin Support Palliat Care. 2015 Jun;9(2):157-62. doi: 10.1097/SPC.0000000000000134. Review.
    PMID: 25872116

  • Network Analysis Reveals Sex- and Antibiotic Resistance-Associated Antivirulence Targets in Clinical Uropathogens

    Parker KS, Wilson JD, Marschall J, Mucha PJ, Henderson JP.
    ACS Infect Dis. 2015 Nov 13;1(11):523-32.

  • “Noncognitive” symptoms of early Alzheimer disease: a longitudinal analysis

    Masters MC, Morris JC, Roe CM.
    Neurology. 2015 Feb. 10; 84(6): 617-22.

  • Restriction of dietary protein decreases mTORC1 in tumors and somatic tissues of a tumor-bearing mouse xenograft model

    Lamming DW, Cummings NE, Rastelli AL, Gao F, Cava E, Bertozzi B, Spelta F, Pili R, Fontana L. Oncotarget. 2015 Oct 13; 6(31): 31233-40. 

  • Interventions to Slow Aging in Humans: Are We Ready? 

    Longo VD, Antebi A, Bartke A, Barzilai N, Brown-Borg HM, Caruso C, Curiel TJ, de Cabo R, Franceschi C, Gems D, Ingram DK, Johnson TE, Kennedy BK, Kenyon C, Klein S, Kopchick JJ, Lepperdinger G, Madeo F, Mirisola MG, Mitchell JR, Passarino G, Rudolph KL, Sedivy JM, Shadel GS, Sinclair DA, Spindler SR, Suh Y, Vijg J, Vinciguerra M, Fontana L. 
    Aging Cell. 2015 Apr 22; 14(4):497-510. 

    Pubmed Abstract 

2014 Publications

  • Energy requirements in nonobese men and women: results from CALERIE

    Redman LM, Kraus WE, Bhapkar M, Das SK, Racette SB, Martin CK, Fontana L, Wong WW, Robert SB, Ravussin E, for the CALERIE Study Group
    Am J Clin Nutr Jan 2014

  • Low protein intake is associated with a major reduction in IGF-1, cancer, and overall mortality in the 65 and younger but not older population

    Levine ME, Suarez JA, Brandhorst S, Balasubramanian P; Cheng CW, Madia F, Fontana L, Mirisola MG, Guevara-Aguirre J, Wan J, Passarino G, Kennedy BK, Wei M, Cohen P, Crimmins EM, Longo, VD
    Cell Metabolism 19, 407-17, March 4, 2014   

  • Optimal body weight for health and longevity: bridging basic, clinical, and population research

    Fontana L, Hu FB
    Aging Cell (2014) pp 1-10

  • Postprandial plasma incretin hormones in exercise-trained versus untrained subjects

    Weiss EP, Royer NK, Fisher JS, Holloszy JO, Fontana L
    Med Sci Sports Exerc 2014 Jun; 46(6): 1098-103

  • Cytochrome P450 gene variants, race and mortality among clopidogrel-treated patients after acute myocardial infarction

    Cresci S, Depta JP, Lenzini PA, Li AY, Lanfear DE, Province MA, Spertus JA,. Bach RG
    Circ Cardiovasc Genet 2014 Jun;7(3):277-86

  • Urinary concentrations of aquaporin-1 and perilipin-2 in patients with renal cell carcinoma correlate with tumor size and stage but not grade

    Morrissey JJ, Mobley J, Song J, Vetter J, Luo J, Bhayani S, Figenshau RS , Kharasch ED
    Urology 2014 Jan;83(1): 256

  • Activation of miR-9 by human papillomavirus in cervical cancer

    Liu W, Gao g,, Hu X, Wang Y, Schwarz JK, Chen JJ, Grigsby PW, Wang X
    Oncotarget, 2014 Nov 30; 5(22):11620-30

  • CHRNA5 variant predicts smoking cessation in patients with acute myocardial infarction

    Chen LS, Bach RG, Lenzini PA, Spertus JA, Bierut LJ, Cresci S
    Nicotine Tob Res 2014 Sep;16(9):1224-31

  • What are the roles of calorie restriction and diet quality in promoting healthy longevity?

    Rizza W, Veronese N, Fontana L
    Ageing Research Reviews 2014 Jan; 13:38-45   

  • Medical research: treat ageing

    Fontana L, Kennedy BK, Longo VD, Seals D, Melov S
    Nature 2014 Jul 24;511(7510):405-7

2013 Publications

  • Amyloid imaging and CSF biomarkers in predicting cognitive impairment up to 7.5 years later

    Roe CM, Fagan AM, Grant EA, Hassenstab J, Moulder KL, Maue Dreyfus D; Sutphen CL, Benzinger TL, Mintun MA, Holtzman DM, Morris JC
    Neurology 2013 May 7;80(19):1784-91

  • The specificity of urinary aquaporin 1 and perilipin 2 to screen for renal cell carcinoma

    Morrissey JJ, Kharasch ED
    The Journal of Urology, 2013 May; 189(5):1913-20

  • Assessing the diagnostic accuracy (DA) of the Spanish version of the informant-based AD8 questionnaire

    Carnero Pardo C, de la Vega Cotarelo R, López Alcalde S, Martos Aparicio C, Vilchez Carrillo R, Mora Gavilán E, Galvin JE
    Neurologia 2013 Mar;28(2):88-94

  • “Deficiency” of mitochondria in muscle does not cause insulin resistance

    Holloszy JO
    Diabetes 2013 Apr;62 (4): 1036-40

  • Calorie restriction in humans inhibits the PI3K/AKT pathway and induces a younger transcription profile

    Mercken EM, Crosby SD, Lamming DW, JeBailey L, Krzysik-Walker S, Villareal DT, Capri M, Franceschi C, Zhang Y, Becker K, Sabatini DM, de Cabo R, Fontana L
    Aging Cell 2013 Aug;12(4):645-51

  • Protein restriction cycles reduce IGF-1 and phosphorylated Tau, and improve behavioral performance in an Alzheimer's disease mouse model

    Parrella E, Maxim T, Maialetti F, Zhang L, Wan J, Wei M, Cohen P, Fontana L, Longo VD
    Aging Cell 2013 Apr;12(2):257-68

  • Identification of a metabolic signature for multidimensional impairment and mortality risk in hospitalized older patients

    Fontana L, Addante F, Copetti M, Paroni G, Fontana A, Sancarlo D, Pellegrini F, Ferrucci L, Pilotto A
    Aging Cell 2013 Jun;12(3):459-66

  • Will calorie restriction work in humans?

    Cava E, Fontana L
    Aging 2013 Jul;5(7):507-14

  • Serum from humans on long-term calorie restriction enhances stress resistance in cell culture

    Omodei D, Licastro D, Salvatore F, Crosby SD, Fontana L
    Aging 2013 Aug;5(8):599-606

  • Energy efficiency as a unifying principle for human, environmental, and global health

    Fontana L, Atella V, Kammen DM
    F1000Research 2013, 2:101

  • Multiple dietary supplements do not affect metabolic and cardiovascular health

    Soare A, Weiss EP, Holloszy JO, Fontana L
    Aging 2013 Sep 4 (Epub)

  • Dietary protein restriction inhibits tumor growth in human xenograft models

    Fontana L, Adelaiye RM, Rastelli AL, Miles KM, Ciamporcero E, Longo VD, Nguyen H, Vessella R, Pili R
    Oncotarget 2013 Dec;4(12):2451-61 

  • Association between specific adipose tissue CD4+ T-cell populations and insulin resistance in obese individuals

    Fabbrini E, Cella M, McCartney SA, Fuchs A, Abumrad NA, Pietka TA, Chen Z, Finck BN, Han DH, Magkos F, Conte C, Bradley D, Fraterrigo G, Eagon JC, Patterson BW, Colonna M, Klein S
    Gastroenterology 2013 Aug;145(2):366-74

  • CSF biomarkers of Alzheimer disease: “noncognitive” outcomes

    Roe CM, Fagan AM, Grant EA, Holtzman DM, Morris JC
    Neurology 2013 Dec 3;81(23):2028-31

  • Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography

    Yoshino J, Imai S
    Methods Mol Biol 2013;1077:203-15

2012 Publications

  • An exploration of salivation patterns in normal weight and obese children

    Aspen VA, Stein RI, Wilfley DE
    Appetite 2012 Apr 58(2):539-42

  • Effect of weight loss on the rate of muscle protein synthesis during fasted and fed conditions in obese older adults

    Villareal DT, Smith GI, Shah K, Mittendorfer B
    Obesity 2012 Sep;20 (9):1780-6

  • Adrenergic-pathway gene variants influence beta-blocker-related outcomes after acute coronary syndrome in a race-specific manner

    Cresci S, Dorn GW 2nd, Jones PG, Beitelshees, AL, Li AY, Lenzini PA, Province MA, Spertus JA, Lanfear DE
    Journal of the American College of Cardiology 2012 Sep 4;60(10):898-907

  • Growth factors, nutrient signaling, and cardiovascular aging

    Fontana L, Vinciguerra M, Longo VD
    Circulation Research 2012 Apr 13;110(8):1139-50

  • Preferential reductions in intermuscular and visceral adipose tissue with exercise-induced weight loss as compared to calorie restriction

    Murphy JC, McDaniel JL, Mora K, Villareal DT, Fontana L, Weiss EP
    Journal of Applied Physiology 2012 Jan;112(1):79-85

  • Klotho locus, metabolic traits, and serum hemoglobin in hospitalized older patients: a genetic association analysis

    Paroni G, Seripa D, Panza F, Addante F, Copetti M, D'Onofrio G, Pellegrini F, Fontana L, Pilotto A
    Age (Dordr) 2012 Aug;34(4):949-68

  • Sexually dimorphic effect of aging on skeletal muscle protein synthesis

    Smith GI, Reeds DN, Hall AM, Chambers KT, Finck BN, Mittendorfer B
    Biology of Sex Differences 2012 May 23;3(1):11

  • Caloric restriction may reverse age-related autonomic decline in humans

    Stein PK, Soare A, Meyer TE, Cangemi R, Holloszy JO, Fontana L
    Aging Cell 2012 Aug;11(4):644-50

  • Dehydroepiandrosterone replacement therapy in older adults improves indices of arterial stiffness

    Weiss EP, Villareal DT, Ehsani AA, Fontana L, Holloszy JO
    Aging Cell 2012 Oct;11(5):876-84

  • Resveratrol supplementation does not improve metabolic function in nonobese women with normal glucose tolerance

    Yoshino J, Conte C, Fontana L, Mittendorfer B, Imai S, Schechtman KB, Gu CC, Kunz I, Rossi Fanelli F, Patterson BW, Klein S
    Cell Metabolism 2012 Nov 7;16(5):658-64

  • [The reliability and validity of Chinese version of AD8]. (article in Chinese)

    Li T, Wang HL, Yang YH, Galvin JE, Morris JC, Yu X
    Zhonghua Nei Ke Za Zhi 2012 Oct;51(10):777-80

  • Predicting road test performance in adults with cognitive or visual impairment referred to a Veterans Affairs Medical Center driving clinic

    Niewoehner PM, Henderson RR, Dalchow J, Beardsley TL, Stern RA, Carr DB
    Journal of the American Geriatric Society 2012 Nov;60(11):2070-4

2011 Publications

  • Ginseng and ginsenoside re do not improve ß-cell function or insulin sensitivity in overweight and obese subjects with impaired glucose tolerance or diabetes

    Reeds DN, Holloszy JO, Patterson BW, Polonsky KS, Okunade A, Klein S
    Diabetes Care Volume 34, May 2011 1071-6

  • Intermittent supplementation with rapamycin as a dietary restriction mimetic

    Longo VD, Fontana L
    Aging (Albany NY) 2011 Nov;3(11):1039-40

  • Application of AD8 questionnaire to screen very mild dementia in Taiwanese

    Yang YH, Galvin JE, Morris JC, Lai CL, Chou MC, Liu CK
    American Journal of Alzheimer’s Disease & Other Dementias 2011 Mar;26(2):134-8

  • Caloric restriction: powerful protection for the aging heart and vasculature

    Weiss EP, Fontana L
    American Journal of Physiology – Heart and Circulatory Physiology 2011 Oct;301(4):H1205-19

  • Regular multicomponent exercise increases physical fitness and muscle protein anabolism in frail, obese, older adults

    Villareal DT, Smith GI, Sinacore DR, Shah K, Mittendorfer B
    Obesity 2011 Feb;19(2):312-8

  • Dietary omega-3 fatty acid supplementation increases the rate of muscle protein synthesis in older adults: a randomized controlled trial

    Smith GI, Atherton P, Reeds DN, Mohammed BS, Rankin D, Rennie MJ, Mittendorfer B
    American Journal of Clinical Nutrition 2011 Feb;93(2):402-12

  • Calorie restriction and prevention of age-associated chronic disease



    Omodei D, Fontana L
    FEBS Letters 2011 Jun 6;585(11):1537-42

  • Reduced bone mineral density is not associated with significantly reduced bone quality in men and women practicing long-term calorie restriction with adequate nutrition



    Villareal DT, Kotyk J,J Armamento-Villareal RC, Kenguva V, Seaman P, Shahar A, Wald MJ, Kleerekoper M, Fontana L
    Aging Cell 2011 Feb; 10(1):96-102

  • Nicotinamide mononucleotide, a key NAD+ intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice.



    Yoshino J, Mills KF, Yoon JY and Imai S.
    Cell Metab. 2011 Oct 5;14(4):528-36.

  • Long-term calorie restriction, but not endurance exercise, lowers core body temperature in humans

    Soare A, Cangemi R, Omodei D, Holloszy JO, Fontana L
    Aging 2011 Apr;3(4):374-9

  • Peroxisome proliferator-activated receptor pathway gene polymorphism associated with extent of coronary artery disease in patients with type 2 diabetes in the bypass angioplasty revascularization investigation 2 diabetes trial

    Cresci S, Wu J, Province MA, Spertus JA, Steffes M, McGill JB, Alderman EL, Brooks MM, Kelsey SF, Frye RL, Bach RG; BARI 2D Study Group
    Circulation 2011 Sep 27;124(13):1426-34

  • Dementia screening, biomarkers and protein misfolding: Implications for public health and diagnosis

    Galvin JE
    Prion 2011 Jan-Mar;5(1):16-21

  • AD8-Brazil: cross-cultural validation of the ascertaining dementia interview in Portuguese

    Correia CC, Lima F, Junqueira F, Campos MS, Bastos O, Petribú K, Laks J, Galvin JE
    Journal of Alzheimer’s Disease.2011;27(1):177-85

  • Dissecting systemic control of metabolism and aging in the NAD World: the importance of SIRT1 and NAMPT-mediated NAD biosysthesis

    Imai S
    FEBS Lett 2011 Jun 6;585(11): 1657-62

  • Predicting road test performance in drivers with dementia

    Carr DB, Barco PP, Wallendorf MJ, Snellgrove CA, Ott BR
    Journal of the American Geriatric Society 2011 Nov;59(11):2112-7

2010 Publications

  • Ten years of NAD-dependent SIR2 family deacetylases: implications for metabolic diseases

    Imai S, Guarente L
    Trends Pharmacol Sci 2010 May;31(5):212-20

  • Relationship of dementia screening tests with biomarkers of Alzheimer's disease

    Galvin JE, Fagan AM, Holtzman DM, Mintun MA, Morris JC
    Brain. 2010 Nov;133(11):3290-300

  • A microRNA expression signature for cervical cancer prognosis

    Hu X, Schwarz JK, Lewis JS Jr, Huettner PC, Rader JS, Deasy JO, Grigsby PW, Wang X
    Cancer Research 2010 Feb 15;70(4):1441-8

  • Extending healthy life span – from yeast to humans

    Fontana L, Partridge L, Longo VD
    Science 2010 Apr 16;328(5976):321-6

  • Long-term effects of calorie restriction on serum sex-hormone concentrations in men

    Cangemi R, Friedmann, AJ, Holloszy JO, Fontana L
    Aging Cell 2010 Apr;9(2):236-42

  • Calorie restriction and cancer prevention: metabolic and molecular mechanisms

    Longo VD, Fontana L
    Trends in Pharmacological Sciences 2010 Feb;31(2):89-98

  • Effects of long-term calorie restriction and endurance exercise on glucose tolerance, insulin action, and adipokine production

    Fontana L, Klein S, Holloszy JO
    Age 2010 Mar;32(1):97–108

  • TRB3 function in cardiac endoplasmic reticulum stress

    Avery J, Etzion S, DeBosch BJ, Jin X, Lupu TS, Beitinjaneh B, Grand J, Kovacs A, Sambandam N, Muslin AJ
    Circulation Research 2010 May 14;106(9):1516-23

  • A possibility of nutriceuticals as an anti-aging intervention: activation of sirtuins by promoting mammalian NAD biosynthesis

    Imai, S
    Pharmacological Research 2010 Jul;62(1):42-7

  • Organizational support and volunteering benefits for older adults

    Tang F, Choi E, Morrow-Howell N
    The Gerontologist 2010 Oct;50(5):603-12

2009 Publications

  • Neuroendocrine factors in the regulation of inflammation: excessive adiposity and calorie restriction

    Fontana L
    Experimental Gerontology 2009 Jan-Feb;44(1-2):41-5

  • “Clocks” in the NAD world: NAD as a metabolic oscillator for the regulation of metabolism and aging

    Imai, S
    Biochimica et Biophysica Acta 2010 Aug;1804(8):1584-90

  • Driving retirement in older adults with dementia

    Croston J, Meuser TM, Berg-Weger M, Grant EA, Carr DB
    Topics in Geriatric Rehabilitation 2009 Apr 1;25(2): 154-62

  • The scientific basis of caloric restriction leading to longer life

    Fontana L
    Current Opinion in Gastroenterology 2009 Mar;25(2):144-50

  • Modulating human aging and age-associated diseases

    Fontana L
    Biochimca et Biophysica Acta 2009 Oct;1790 (10):1133-8

  • SIRT1 and caloric restriction: an insight into possible trade-offs between robustness and frailty

    Imai S
    Current Opinion in Clinical Nutrition and Metabolic Care 2009 Jul;12(4):350-6

  • Who benefits from volunteering: Variations in perceived benefits

    Morrow-Howell N, Hong SI, Tang F
    The Gerontologist 2009 Feb;49 (1):91-102

  • Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis

    Ramsey KM, Yoshino J, Brace CS, Abrassart D, Kobayashi Y, Marcheva B, Hong HK, Chong JL, Buhr ED, Lee C,Takahashi JS, Imai S, Bass J
    Science 2009 May 1;324(5927): 651-4

2008 Publications

  • MAPK signalling in cardiovascular health and disease: molecular mechanisms and therapeutic targets

    Muslin AJ
    Clinical Science (London, England) 2008 Oct;115(7):203-18

  • The anticonvulsant ethosuximide disrupts sensory function to extend C. elegans lifespan

    Collins JJ, Evason K, Pickett CL, Schneider DL, Kornfeld K
    PLoS Genetics 2008 Oct;4(10): e1000230

  • The measurement and analysis of age-related changes in Caenorhabditis elegans

    Collins JJ, Huang C, Hughes S, Kornfeld K
    WormBook 2008 Jan 24:1-21

  • Reduction of overweight and eating disorder symptoms via the Internet in adolescents: A randomized controlled trial

    Doyle AC, Goldschmidt A, Huang C, Winzelberg AJ, Taylor CB, Wilfley DE
    Journal of Adolescent Health 2008 Aug;43 (2): 172-9

  • Valproic acid extends Caenorhabditis elegans lifespan

    Evason K, Collins JJ, Huang C, Hughes S, Kornfeld K
    Aging Cell 2008 Jun;7(3):305-17

  • Long-term effects of calorie or protein restriction on serum IGF-1 and IGFBP-3 concentration in humans

    Fontana L, Weiss EP, Villareal DT, Klein S, Holloszy JO
    Aging Cell 2008 Oct;7(5):681-7

  • Translational approaches to addressing complex genetic pathways in colorectal cancer

    Early DS, Fontana L, Davidson NO
    Translational Research 2008 Jan;151(1):10-6

  • Calorie restriction and cardiometabolic health

    Fontana L
    European Journal of Cardiovascular Prevention and Rehabilitation 2008 Feb;15(1):3-9

  • Changes in weight and health behaviors from freshman through senior year of college

    Racette, SB, Deusinger SS, Strube MJ, Highstein GR, Deusinger RH
    J Nutr Educ Behav 2008 Jan-Feb;40(1):39-42

2007 Publications

  • Long-term low-calorie low-protein vegan diet and endurance exercise are associated with low cardiometabolic risk

    Fontana L, Meyer TE, Klein S. Holloszy JO
    Rejuvenation Res 2007 Jun;10(2):225-34

  • Aging, adiposity and calorie restriction

    Fontana L, Klein S
    JAMA 2007 Mar 7;297(9):986-94

  • Evaluation of cognitive impairment in older adults: combining brief informant and performance measures

    Galvin JE, Roe CM, Morris JC
    Archives of Neurology 2007 May,64(5):718-24

  • Patient’s rating of cognitive ability: using the AD8, a brief informant interview, as a self-rating tool to detect dementia

    Galvin JE, Roe CM, Coats MA, Morris JC
    Archives of Neurology 2007 May;64(5):725-30

  • Quantifying the usefulness of CT in evaluating seniors with abdominal pain

    Lewis LM, Klippel AP, Bavolek RA, Ross LM, Scherer TM, Banet GA
    European Journal of Radiology 2007 Feb;61(2):290-6

  • Productive engagement and late life physical and mental health: Findings from a nationally representative panel study

    Hinterlong JE, Morrow-Howell N, Rozario PA
    Research on Aging 2007 Jul;29(4):348-70

2006 Publications

  • Effects of anticonvulsant drugs on life span

    Kornfeld K, Evason K
    Archives of Neurology 2006 Apr;63(4):491-6

  • Pharmacology of delayed aging and extended lifespan of Caenorhabditis elegans

    Collins JJ, Evason K, Kornfeld K
    Experimental Gerontology 2006 Oct;41(10):1032-9

  • Validity and reliability of the AD8 informant interview in dementia

    Galvin JE, Roe CM, Xiong C, Morris JC
    Neurology 2006 Dec 12;67(11):1942-8

  • Effect of lifestyle intervention on metabolic coronary heart disease risk factors in obese older adults

    Villareal DT, Miller BV III, Banks M, Fontana L, Sinacore DR, Klein S
    The American Journal of Clinical Nutrition 2006 Dec;84(6):1317–23

2005 Publications

  • Anticonvulsant medications extend worm life-span

    Evason K, Huang C, Yamben I, Covey DF, Kornfeld K
    Science 2005 Jan 14;307(5707):258-62

  • The AD8: a brief informant interview to detect dementia

    Galvin JE, Roe CM, Powlishta KK, Coats MA, Muich SJ, Grant E, Miller JP, Storandt M, Morris JC
    Neurology 2005 Aug 23;65(4):559-64

  • Obesity in older adults: Technical review and position statement of the American Society for Nutrition and NAASO, The Obesity Society

    Villareal DT, Apovian CM, Kushner RF, Klein S
    Obesity Research 2005 Nov;13(11):1849-63

2003 Publications

  • Effects of volunteering on the well-being of older adults

    Morrow-Howell N, Hinterlong J, Rozario PA, Tang F
    The Journal of Gerontology Series B, Psychological Sciences and Social Sciences. 2003 May;58(3):S137-45

  • Predictors of mortality in younger and older patients with heart failure and preserved or reduced left ventricular ejection fraction

    Kerzner R, Gage BF, Freedland KE, Rich MW
    American Heart Journal 2003 Aug;146(2):286-90

  • Identifying factors associated with disability-related differences in breast cancer screening (United States)

    Schootman M, Jeffe DB
    Cancer Causes and Control 2003 Mar;14(2):97-107